产品简介

K-562是人慢性髓性白血病(Chronic Myelogenous Leukemia，CML)细胞，源自一位临终的53岁女性慢性髓性白血病患者，从患者的胸水中分离获得，由Lozzio建立。K-562属于高度未分化的粒性白细胞。Anderson等对其表面膜性质的研究证实K-562是一株人类红白血病细胞。K-562细胞株在自然杀伤研究分析中被广泛用作高敏感的体外受体。See Pross等将它用于NK细胞的体外检测，包括NK细胞活性的数学量化。K-562母细胞是多能性，造血恶性细胞，它能自发分化成可识别的红血球祖细胞、粒性白细胞、单核细胞等。

K-562细胞系(K-562 Cell Line)为人慢性髓性白血病细胞，悬浮生长，培养条件为含10%FBS的IMDM或10%FBS的RPMI-1640完全培养液。

包装

保存

无菌包装。完全培养液或液氮冻存。

参考文献

1、Jondal M, Pross H. Surface markers on human b and t lymphocytes. VI. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations. Int. J. Cancer 15: 596-605, 1975. PubMed: 806545

2、Lozzio CB, Lozzio BB. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45: 321-334, 1975. PubMed: 163658

3、Ortaldo JR, et al. Specificity of natural cytotoxic reactivity of normal human lymphocytes against a myeloid leukemia cell line. J. Natl. Cancer Inst. 59: 77-82, 1977. PubMed: 69036

4、West WH, et al. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells. J. Immunol. 118: 355-361, 1977. PubMed: 299761

5、Lozzio BB, Lozzio CB. Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Leuk. Res. 3: 363-370, 1979. PubMed: 95026

6、Andersson LC, et al. K562--a human erythroleukemic cell line. Int. J. Cancer 23: 143-147, 1979. PubMed: 367973