产品编号
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包装
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价格
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6510017-L
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1瓶
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1200.00
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产品简介
L929为小鼠成纤维细胞,是最早建立的连续培养物之一。L929源自100天的C3H/An雄性小鼠乳晕和脂肪等皮下结缔组织(Subcutaneous Connective Tissue)。该细胞系生长稳定,繁殖旺盛,细胞株的生物学特征符合成纤维细胞的生物学特征,细胞群体倍增时间约26小时。常用于药品、医疗器械、医疗用品等的细胞毒和细胞增殖实验。
L929细胞系(L929 Cell Line)为小鼠成纤维细胞,纤维母细胞样贴壁生长,培养条件为含10%FBS 的MEM完全培养液或10%FBS 的RPMI-1640完全培养液。细胞分散为胰酶EDTA细胞消化液。
包装
产品编号
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6510017-L
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L929细胞系
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1瓶
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说明书
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1份
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保存
无菌包装。完全培养液或液氮冻存。
参考文献
1、Sanford KK, et al. The growth in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 9: 229-246, 1948
2、Westfall BB, et al. The glycogen content of cell suspensions prepared from massive tissue culture: comparison of cells derived from mouse connective tissue and mouse liver. J. Natl. Cancer Inst. 14: 655-664, 1953. PubMed: 13233820
3、Earle WR, et al. Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J. Natl. Cancer Inst. 4: 165-212, 1943.
4、Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002.
5、Plastics collapsible containers for human blood and blood components--Part 1: Conventional containers. Annex C. Biological tests.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 3826-1:2003.
6、Dentistry--Preclinical evaluation of biocompatibility of medical devices used in dentistry--Test methods for dental materials. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 7405:1997.
7、Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999.
8、Plastic containers for intravenous injection. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 15747:2003.
9、医疗器械生物学评价第五部分:体外细胞毒实验 GB/T_16886.5 2003
10、医用输液、输血、注射器具检验方法第2部分:生物学试验方法 BG/T_14233.2 2005
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