产品简介

    MDA-MB-453是人乳腺癌细胞， 源自一位48岁女性乳腺癌患者。1976年，R，Cailleau等从患者的心包积液(Pericardial Effusion)中分离出肿瘤细胞，经体外连续传代培养建系。MDA-MB-453细胞过表达成纤维细胞生长因子受体（Fibroblast Growth Factor Receptor，FGFR)，并可在半固态培养基上生长。该细胞系生长稳定，繁殖旺盛，扩增传代比例为1:2-6。

    MDA-MB-453细胞系(MDA-MB-453 Cell Line)为人乳腺腺癌细胞，上皮样贴壁生长，培养条件为含10%FBS的L-15(Leibovitzs L-15 Medium)完全培养液。细胞分散为胰酶EDTA细胞消化液。使用L-15培养基时，CO2对细胞有害，请勿在CO2培养箱中培养。 

包装

保存

无菌包装。完全培养液或液氮冻存。

参考文献

1、Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed:730202

2、Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed:427779

3、Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed:7000337

4、McLeskey SW, et al. MDA-MB-134 breast carcinoma cells overexpress fibroblast growth factor (FGF) receptors and are growth-inhibited by FGF ligands. Cancer Res. 54: 523-530, 1994. PubMed:7506125