产品简介

PC-12是大鼠肾上腺嗜铬细胞瘤(Pheochromocytoma)。该细胞系来自雄性大鼠(Rattus Norvegicus, Rat)的肾上腺嗜铬细胞瘤，表达神经生长因子(Nerve Growth Factor，NGF)受体。NGF可诱导该细胞产生可逆性的神经表型。该细胞可产生儿茶酚胺(Catecholamines)、多巴胺(Dopamine)和去甲肾上腺素(Norepinephrine，NE)，但不合成肾上腺素(Epinephrine)。该细胞系生长稳定，繁殖旺盛，细胞群体倍增时间48小时。

PC-12细胞系(PC-12 Cell Line)为大鼠肾上腺嗜铬细胞瘤细胞，贴壁疏松，易聚集生长，培养条件为含10% 灭活马血清+5% FBS的RPMI-1640完全培养液。细胞分散一般不需要细胞消化液。

包装

保存

无菌包装。完全培养液或液氮冻存。

参考文献

1、Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

2、Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712

3、Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed:3839317

4、Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125

5、U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005