产品简介

NFkB （DNA binding factor KBF2，H2TF1，NFKB-p52，Lymphocyte translocation chromosome 10，Lyt 10，Lyt10，NFKB2，Nuclear factor NF kappa B p100 subunit，Nuclear factor NF kappa B p52 subunit，Nuclear factor of kappa light polypeptide gene enhancer in B cells 2; Oncogene Lyt 10，p49/p100.）is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.

兔抗NF-κB2多克隆抗体(Rabbit Anti-NF-κB2 Polyclonal Antibody, Nuclear factor NF kappa B p52 subunit，NF-κB2，NFKB-p52,NFKBp52）是以NF-κB2的合成多肽与KLH偶联，免疫健康家兔，获得高效价免疫血清，再经蛋白A亲和柱层析纯化，溶于PBS （pH7.4）中，蛋白浓度1mg/ml，可广泛用于NF-κB2的各种免疫检测分析。

包装

保存

在-20℃或以下可保存1年或以上。使用过程中可短时间保存于4℃。请勿反复冻融或于0℃以上久置。

使用说明

本产品经一抗稀释液稀释后可用于ELISA（1:1000），IHC（1:200），IP（1:50），WB（1:300）等免疫检测分析。