产品简介

The transcriptional nuclear factor kappa B (NF-κB) plays a pivotal role in inflammatory and immune responses. NF-κB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NF-κB1) subunits. Other identified subunits include p52 (NF-κB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NF-κB subunit p65, similar to p50/p65 heterodimers. NF-κB is composed of a heterodimer of p65 and p50 subunits in most cell types and is sequestered in the cytoplasm by its inhibitory proteins, the IκBs. IκB-α binds to the p65 subunit (nuclear localization sequence, NLS) and IκB-α binding masks the NF-κB nuclear localization sequence (NLS), preventing NF-κB nuclear localization and DNA binding. NF-κB activating agents can induce the phosphorylation of IκBs; the phosphorylation targets IκBs for rapid degradation through an ubiquitin-proteasome pathway, thereby releasing NF-κB to enter the nucleus for gene expression. It has been shown that the p65 NF-κB subunit is also phosphorylated during the phosphorylation and degradation of IκBs; the phosphorylation site is Ser536 in the C-terminal transactivation domain. Phosphorylation of NF-κB p65 at serine 276 allows for increased intrection with the transcriptional coactiveator p300/CBP to further enhance the transcriptional activeity of this transcription factor.

兔抗RelA多克隆抗体（Rabbit Anti-RelA Polyclonal Antibody,RelA，NFKB3）是以RelA的合成多肽与KLH偶联，免疫健康家兔，获得高效价免疫血清，再经蛋白A亲和柱层析纯化，溶于PBS（pH7.4）中，蛋白浓度1mg/ml，可广泛用于RelA的各种免疫检测分析。

包装

保存

在-20℃或以下可保存1年或以上。使用过程中可短时间保存于4℃。请勿反复冻融或于0℃以上久置。

使用说明

本产品经一抗稀释液稀释后可用于ELISA（1:1000），IHC（1:200），IP（1:50），WB（1:300）等免疫检测分析。